HBMECs were isolated from normal human brain tissue. Passage 2, cells are ship on dry ice in a vial (also available proliferating culture with a confluency of >90 %, HEC02-T25). ENDO-Growth Medium (MED001) is recommended for culture. Cells have an average additional population doubling levels >15 when cultured.

Characterization of the cells

• Cytoplasmic VWF / Factor VIII: >95% positive by immunofluorescence

• Cytoplasmic uptake of Di-I-Ac-LDL: >95% positive by immunofluorescence

• Cytoplasmic PECAM1 >95% positive by immunofluorescence 

HBMECs are negative for HIV-1, HBV, HCV, and mycoplasma.

Date of Expiration: Guaranteed for up to 3 months from date of shipping when stored in liquid nitrogen. We guarantee these cells when cultured according to our protocol and with our coating solution and media.