[Abbkine]PurKine™MBP-Tag Dextrin Packed Column 6FF
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Product namePurKine™ MBP-Tag Dextrin Packed Column 6FF
Application notesPurKine™ MBP-Tag Dextrin Resin 6FF effectively purifies high levels of overexpressed MBP-tagged fusion proteins in gravity column procedures at a variety of scales. The Resin consists of 90μm beads of highly cross-linked 6% agarose, to which Dextrin has been coupled. Tests confirm that performance equals or exceeds popular Dextrin resins from other suppliers, and no decrease in performance occurs after at least five repeated uses. In addition, its high flow properties make it excellent for scaling-up.
FormulationPrepacked columns prepacked with 50% slurry in 20% ethanol.
Features & Benefits• High capacity—More than 10 mg of MBP-tagged protein per milliliter of resin.
• High performance—Physiological conditions and mild elution is performed using maltose which preserve target protein activity.
• Cost-effective—No performance decrease after at least five repeated uses of the same batch of resin.
• Flexible—available in multiple formats including bulk resin, spin columns and complete kits.
• Robust—highly crosslinked beads tolerate linear flow rates up to 500 cm/hour.
Storage bufferLiquid resin in PBS buffer containing 20% ethanol were prepacked in columns.
Storage instructionsStable for one year at 2-8°C from date of shipment. Do not freeze.
PrecautionsThe product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
BackgroundPurKine™ MBP-Tag Purification system is based on innovative high-capacity matrix for convenient single-step purification of recombinant maltose binding proteins (MBP-tagged protein). Recombinant proteins with MBP-tags often gives increased expression levels and higher solubility of the target protein. Proper folding of the attached protein has also been shown to be promoted by the MBP tag. Since MBP increase the solubility, the tag is extremely useful for recombinant proteins accumulated in an insoluble form (inclusion bodies). The portfolio allow optimization of process for maximum protein yield, stability and solubility. Tests also confirm that no decrease in performance occurs after at least five repeated uses. The PurKine™ MBP-Tag Purification resin is also available as prepacked spin column and kit formats.
Alternative namesDextrin ; MBP resin; Dextrin resin; MBP tag resin
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