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[neuromics] HUMAN BRAIN MICROVASCULAR ENDOTHELIAL CELLS

작성자 관리자 작성일 2020-02-25
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HUMAN BRAIN MICROVASCULAR ENDOTHELIAL CELLS

 

Product Details

CATALOG NUMBER: HEC02

APPLICATIONS: Cell Assays

TYPE: Primary Cells

STORAGE: Keep frozen in liquid nitrogen until plating

SHIPPING: Frozen (Dry Ice/Liquid Nitrogen)

SPECIES REACTIVITY: Human

 

 

HBMECs were isolated from normal human brain tissue. Passage 2, cells are ship on dry ice in a vial (also available proliferating culture with a confluency of >90 %, HEC02-T25). ENDO-Growth Medium (MED001) is recommended for culture. Cells have an average additional population doubling levels >15 when cultured.

Characterization of the cells

  • Cytoplasmic VWF / Factor VIII: >95% positive by immunofluorescence

  • Cytoplasmic uptake of Di-I-Ac-LDL: >95% positive by immunofluorescence

  • Cytoplasmic PECAM1 >95% positive by immunofluorescence 

HBMECs are negative for HIV-1, HBV, HCV, and mycoplasma.

Date of Expiration: Guaranteed for up to 3 months from date of shipping when stored in liquid nitrogen. We guarantee these cells when cultured according to our protocol and with our coating solution and media. 

Supporting Products
NameCatalog #Size
1X Phosphate Buffer SolutionPBS300100 ml
AlphaBioCoat SolutionAC00120 ml
Cell Detachment SolutionADF00150 ml
ENDO Basal MediaMED002500 ml
ENDO-Growth MediaMED001500 ml
ENDO-Growth Media KitEGK001500 ml
ENDO-Growth SupplementsEGS00125 ml
Endothelial Cryopreserve MediaFM30010 ml
Smooth Coat SolutionSC30020 ml

 

 

 

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