Development of a human corneal epithelium model utilizing a collagen v…
작성자 | 관리자 | 작성일 | 2023-02-24 |
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Development of a human corneal epithelium model utilizing a collagen vitrigel membrane and the changes of its barrier function induced by exposing eye irritant chemicals
Highlights
► A human corneal epithelium model was reconstructed on a collagen vitrigel membrane. ► Thin collagen vitrigel membrane was used as a substitute for Bowman’s membrane. ► The model appropriate for TEER assay was composed of 5–6 HCE-T cell layers. ► The changes of epithelial barrier function after exposing chemicals were measured. ► The brief TEER assay using the model was useful for an ocular irritancy evaluation.
Abbreviations
BCOP
bovine corneal opacity and permeability
ICE
isolated chicken eye
IRE
isolated rabbit eye
SIRC
Statens Serum Institut rabbit corneal
STE
short time exposure
TEER
trans-epithelial electrical resistance
DMEM
Dulbecco’s modified Eagle Medium
F-12
nutrient mixture F-12
FBS
fetal bovine serum
EGF
epidermal growth factor
DMSO
dimethyl sulfoxide
PET
polyethylene terephthalate
ERS
electrical resistance system
GHS
globally harmonized system
NI
non-irritant
EDTA
ethylenediaminetetraacetic acid
PBS
phosphate buffered saline
ADMET
absorption, distribution, metabolism, excretion and toxicity
Keywords
Corneal epithelium
Eye irritation tests
Collagen vitrigel membrane
TEER
HCE-T cells